Improved Thermodynamic Parameters and Helix Initiation Factor to Predict Stability of DNA Duplexes
نویسندگان
چکیده
منابع مشابه
Improved thermodynamic parameters and helix initiation factor to predict stability of DNA duplexes.
To improve the previous DNA/DNA nearest-neighbor parameters, thermodynamic parameters (deltaH degrees, deltaS degrees and deltaG degrees) of 50 DNA/DNA duplexes were measured. Enthalpy change of a helix initiation factor is also considered though the parameters reported recently did not contain the factor. A helix initiation factor for DNA/DNA duplex determined here was the same as that of RNA/...
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Thermodynamic parameters of helix-coil transitions in polypeptide chains.
The aim of this paper is to outline new possibilities of more detailed thermodynamic analysis of helix coil transition parameters, particularly with respect to their splitting into energy and entropy terms. Because of difficulties with the ternary systems, water—organic solvent polypeptide, or, two organic solvents polypeptide, caused particularly by the phenomenon of specilic sorption of one s...
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Locked nucleic acids (LNA; symbols of bases, +A, +C, +G, and +T) are introduced into chemically synthesized oligonucleotides to increase duplex stability and specificity. To understand these effects, we have determined thermodynamic parameters of consecutive LNA nucleotides. We present guidelines for the design of LNA oligonucleotides and introduce free online software that predicts the stabili...
متن کاملImproved nearest-neighbor parameters for predicting DNA duplex stability.
Thermodynamic data were determined from UV absorbance vs temperature profiles of 23 oligonucleotides. These data were combined with data from the literature for 21 sequences to derive improved parameters for the 10 Watson-Crick nearest neighbors. The observed trend in nearest-neighbor stabilities at 37 degrees C is GC > CG > GG > GA approximately GT approximately CA > CT > AA > AT > TA (where o...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 1996
ISSN: 1362-4962,0305-1048
DOI: 10.1093/nar/24.22.4501